Coronaviruses Methods and protocols

Coronaviruses: Methods and Protocols (Methods in Molecular Biology)
Editor: Humana Press  Language: English    Pages: 299

Series:
Methods in Molecular Biology (

Book file:
PDF, 15,36 MB

This volume aims to describe a variety of techniques that reflect the wide range of research currently being conducted in the field of coronavirology, and begins with an overview of current knowledge on replication and pathogenesis of coronaviruses to introduce specialists and non-specialists in the domain. The rest of the book is divided into several sections of chapters starting with those that describe the identification, diagnosis and study of the evolution of coronaviruses. The following chapters deal with the preparation of cell and organ cultures useful for the propagation of coronaviruses and titration techniques, as well as techniques for the analysis of viral functions which require purification of the viruses. The following chapters describe two reverse genetics techniques commonly used for coronaviruses, and techniques detailing the identification of cellular receptors, viral binding protein binding profiles, and cell-virus fusion. The final chapters cover a wide range of techniques for identifying virus-protein-protein interactions, confirming the functional role of these proteins in virus replication, studying host cell responses through genome-wide approaches or specific. to the pathway, and visualize the virus replication complexes. Written in the highly successful format of the Molecular Biology Methods series, the chapters include the type of detailed description and implementation tips that are essential for optimal laboratory results. study host cell responses through genome-wide or path-specific approaches and visualize virus replication complexes. Written in the highly successful format of the Molecular Biology Methods series, the chapters include the type of detailed description and implementation tips that are essential for optimal laboratory results. study host cell responses through genome-wide or path-specific approaches, and visualize virus replication complexes. Written in the highly successful format of the Molecular Biology Methods series, the chapters include the type of detailed description and implementation tips that are essential for optimal laboratory results.