Description

This Master Prompt provides a comprehensive, advanced and methodologically rigorous framework for evaluating diagnostic accuracy, biomarker discrimination, classification performance and clinically meaningful decision thresholds using GraphPad Prism. It integrates diagnostic research design, quantitative biomarker measurement, reference-standard classification, data preparation, receiver operating characteristic (ROC) analysis, area under the curve (AUC) estimation, sensitivity, specificity, diagnostic threshold selection, statistical uncertainty and scientific interpretation into a unified analytical workflow.